At the same time, we detected the expression of CST1 and GPX4 proteins in normal intestinal mucosal epithelial cells HIEC and colon cancer cell lines HCT116 etc., the results showed that the protein levels of CST1 and GPX4 were significantly increased in colon cancer cells, while their expression was lower in HIEC. Here, CST1 is linked to malignant colon neoplasm.