The prostate cancer cell lines that we tested included two C57BL/6 syngeneic mouse prostate cancer cells [RM-128 and a Pten/Kras line that we established from the mouse model with prostate specific Pten deletion and KrasG12D activation29 (Supplementary Fig. 2c)] and three human prostate cancer cell lines (PC3, DU145, and LnCaP cells). Here, PTEN is linked to Familial prostate cancer.