SYP and adenocarcinoma: Immunostaining of synaptophysin and qRT-PCR analysis of the NEPC-related genes (Syp, Chga, and Eno2) in the adenocarcinoma of different lobes also showed no significant changes in NEPC cell frequency (Supplementary Fig. 5b) or NEPC gene expression (Supplementary Fig. 5c), respectively.