The quantitative analysis indicates a significant increases in the mean intensity fluorescence (MFI) of thrombin by 254.1 ± 41.1%, IL-8/CXCL8 by 245.7 ± 35.0%, and DCLK1 by 212.4 ± 31.0% in severe asthma patients compared to normal (Fig. 2C). The gene discussed is CXCL8; the disease is asthma.