Semiquantitative analysis indicated that the levels of H2A.Z expression in the ICC tissues were significantly higher than those in the nontumor tissues (7.15 ± 0.43 vs. 1.52 ± 0.28, ***p < 0.001), while the expression level of SFRP1 was significantly lower in the ICC tissues than in the nontumour tissues (1.82 ± 0.58 vs. 4.85 ± 0.30, *p < 0.05; Fig. 1B). The gene discussed is SFRP1; the disease is intrahepatic cholangiocarcinoma.