To understand whether there is a regulatory relationship between H2A.Z and SFRP1, we first determined the relative levels of H2A.Z expression in HuccT-1, HCCC-9810 and RBE ICC cells as well as the nontumor cholangiocyte line HIBEpic by quantitative RT–PCR and Western blot analysis. This evidence concerns the gene SFRP1 and intrahepatic cholangiocarcinoma.