We divided the following main clusters LUSC (KRT5, KRT6A, KRT17, SOX2, NTRK2, TP63); LUAD (CD24, MUC1, KRT7, NAPSA, NKX2-1, MSLN); LUAD with signs of epithelial-to-mesenchymal transition (LUAD EMT) (VIM, SERPINE1, CDH1, MIF); LUAD with neuroendocrine features (LUAD NE) (CHGA, SYP, NCAM1, TUBA1A); LUAD with high expression of mesothelin (MSLN) associated with EMT and metastasis (LUAD MSLN);23 and NSCLC expressing both LUAD and LUSC markers (NSCLC mixed) (MUC1, KRT7, KRT6A, SOX2) (Figures 1B and S1D). The gene discussed is NKX2-1; the disease is non-small cell lung carcinoma.