Though the diminution of EMT transcription factors, Snail and Slug, as well as anti-apoptotic Bcl-2 and Mcl-1 proteins was not obviously observed in detached lung cancer cells cultured with anti-PD-L1 at low concentration (Fig 8A and 8C), the dramatic up-regulation of N-cadherin expression level (Fig 8D) and % cell viability (Fig 7A) was demonstrated in the cells incubated with 0.1 μg/mL anti-PD-L1 for 12 h. This evidence concerns the gene CD274 and lung cancer.