The results showed that NH4Cl significantly limited Wa infection at early stage, and Wa escaped the NH4Cl blockade at around 120 min in Caco2–ΔIFITM3 cells, while Wa infection was still significantly restricted at the same time in Caco2 cells, which indicated that IFITM3 may delay the kinetics of RV endosomal escape. Here, IFITM3 is linked to infection.