In the majority of NAFLD patients, ACE2 positivity was detectable in both hepatocytes and and bile ducts, concording with recently reported data [23] while TMPRSS2 positivity was present in both the cytoplasm and nuclei of hepatocytes although the number of positive nuclei differed among different areas of the same biopsy (Figure 4). Here, TMPRSS2 is linked to metabolic dysfunction-associated steatotic liver disease.