To examine the effect of Bub3 on cell-cycle arrest, cells pre-transfected with RNAi-Ctrl or RNAi-Bub3 were transfected with pRK5-Flag-p17 or infected with ARV at an MOI of 1, followed by flow cytometry analysis at 24, 48 and 72 h post infection. This evidence concerns the gene BUB3 and infection.