Confocal laser scanning microscopy was applied to investigate the potential of CDC-H for cell imaging and the cellular uptake of DOX–CDC-H in different cells (NIH-3T3 and 4T1 cells), and the results confirmed the target cell imaging and cellular uptake of DOX–CDC-H by specifically binding the CD44 receptors on the surface of tumor cells. Here, CDK20 is linked to neoplasm.