Delivery of silencing miCTGF RNA by cationic microbubble–liposome complexes downregulated CTGF gene expression in HSC-T6 cells in vitro and significantly ameliorated fibrotic factors CTGF, TGFβ1, collagen I, and α-SMA expression in the rat liver in a fibrosis model in vivo. Here, CCN2 is linked to fibrosis.