These results suggest that the Bcl2 gene is modestly expressed (i.e., not at an extreme level among the selected biomarkers analyzed) in the human colon cancer cells treated with gossypol, LPS, and cottonseed extracts and that the Bcl2 gene could serve as a good reference gene for qPCR analysis of gene expression in the human colon cancer cells. This evidence concerns the gene BCL2 and colonic neoplasm.