This could be due to the PI3K signaling pathway’s signaling cascade amplification effect, which causes mTOR expression instability, but the combined phosphorylated AKT and ERK inhibition results could already indicate that 3039-0164 inhibited the activation of the PI3K/AKT/mTOR and ERK pathways in NSCLC cells. Here, AKT1 is linked to non-small cell lung carcinoma.