The results of animal experiments showed that AGA could induce apoptosis of H22 tumor cells in vivo by improving the status of immune organs, increasing the phagocytic ability of peritoneal macrophages, the killing power of NK cells and the proliferation ability of lymphocytes, increasing the levels of cytokines (TNF-α, IL-2, IL-4 and IFN-γ), and regulating the distribution of lymphocyte subpopulations, thus achieving anti-tumor effects in vivo. This evidence concerns the gene TNF and neoplasm.