CD34 and neoplasm: To explore further the related mechanism of 2′-FL in suppressing HCT116 tumors, we conducted HE staining and CD34 staining of tumor tissue, and found that the numbers of newborn blood vessels in tumors treated with 5-Fu, 2′-FL, or the co-treatment groups were less than the control level (Figure 4A), and the ones in the co-treatment group were the smallest (Figure 4B); besides, the edematous and hemorrhage areas in the treatment groups were also smaller when compared with the control group (Figure 4A).