In Azagra-Boronat’s study, animals given 2′-FL showed higher plasma IgG and IgA and more T cell subsets in the mesenteric lymph nodes on day 16, indicating that 2′-FL might have anti-cancer effects through immunomodulatory and prebiotic pathways [27]; thus, in the present study, we evaluated the anti-tumor potentials of 2′-FL in a xenograft colon tumor model and explored the molecular mechanism on how 2′-FL exerted effects in inhibiting colon cancer. Here, CD79A is linked to colonic neoplasm.