We disclosed that PEPE2 fraction from the ethyl acetate extract of pomegranate could inhibit the bladder tumor by regulating (1) the HSP90-associated pathway, which regulates the cell proliferation; (2) PTP1B, which is a tumor suppressor; (3) apoptotic genes; and (4) cytoskeleton assembly. This evidence concerns the gene HSP90AA1 and urinary bladder neoplasm.