To examine whether programmed cell death of PCa caused by AIF was due to the activation of caspase-dependent apoptosis, we first measured the population numbers (%) of apoptotic cells in LNCaP and C4-2 cells after AIF treatment assayed by Annexin V-Fluorescein isothiocyanate (FITC)/propidium iodide (PI) staining with flow cytometry analysis. Here, ANXA5 is linked to posterior cortical atrophy.