Western blot (WB) results showed that, compared to the Sham group, FBXO3 protein expression was increased after 6 h of reperfusion and peaked at 24 h post-stroke (Sham group vs. MCAO 1 h/R 24 h group, p < 0.01) (Figure 1A,B), so these conditions were chosen for subsequent experiments. The gene discussed is FBXO3; the disease is Stroke.