In order to observe the level of adiponectin protein in murine hepatocytes, immunohistochemistry (IHC) analysis was performed on liver tissue sections from Leprdb mice (T2DM group) and Dock7m mice (control group) to quantify and compare the staining results of mice with different disease courses (4, 16, and 32 w). The gene discussed is ADIPOQ; the disease is type 2 diabetes mellitus.