We further found that the protein expression levels of senescence-related molecules p53, p16, p21, and Sirt1 were upregulated and that α-Klotho, a putative aging-suppressor protein, was downregulated in the kidneys of the IRI-induced CKD mice, which could be reversed by LIPUS treatment (Figure 3D). This evidence concerns the gene TP53 and chronic kidney disease.