Functionally, C-Kit overexpression promoted stemness in CRC and significant resistance to bufalin’s anti-stemness effect in matrigel spheroid assay (p = 0.0306; Figure 3D) To ascertain the role of Slug in this process, we overexpressed Slug in the DLD1 cell line, which has low endogenous expression of this protein and a more epithelial appearance compared to HCT116 and SW480 cell lines, which have previously been classified in the high EMT CMS4 CRC subtype [46]. This evidence concerns the gene SNAI2 and colorectal carcinoma.