In CRC with lymph node metastases, there was 51.4-fold upregulation of PDGFD (p ≤ 0.01), 2.7-fold upregulation of CKD1 (p ≤ 0.05), and 7.9-fold upregulation (p ≤ 0.05) of ANLN. The results are shown in Figure 7c. This evidence concerns the gene PDGFD and metastatic malignant neoplasm in the lymph nodes.