Implementation of a DAVID Functional Annotation Chart (https://david.ncifcrf.gov/tools.jsp, accessed on 4 July 2022) using the horse genome as the background showed significant enrichment of sets of genes involved in pathways in cancer (ecb05200), cell cycle (ecb04110), focal adhesion (ecb04510), ECM–receptor interaction (ecb04512), glycolysis/gluconeogenesis (ecb00010), proteoglycans in cancer (ecb05205), HIF-1 signaling pathway (ecb04066), PI3K-Akt signaling pathway (ecb04151), FoxO signaling pathway (ecb04068), and TGF-beta signaling pathway (ecb04350) (Table S7–S10). This evidence concerns the gene AKT1 and cancer.