CBD induced colorectal cancer cell apoptosis by the activation of the ROS-mediated ER-stress pathway and its downstream regulators (p-eIF2 α, ATF3, ATF4, CHOP), which is reversible by adding the ROS scavenger, N-acetyl cysteine, or using CHOP small interfering RNA (siRNA) [12,49]. This evidence concerns the gene DDIT3 and colorectal cancer.