To identify the underlying reason for the delayed onset and the reduced amount of cytokines present in plasma of SphK1/2 deficient mice post infection, we investigated the cytokine release of bone marrow-derived macrophages (BMDM), T cells and B cells isolated from spleen, and mouse lung endothelial cells (MLEC) isolated from lung of wt, SphK1−/− and SphK2−/− mice. This evidence concerns the gene SPHK1 and infection.