As a widely used CNS transduction vector, AAV9 exhibits efficient infection of neurons and high abundance of gene expression, so AAV13-hSyn-Cre as a “controller” mixed with AAV9-CAG-DIO-EGFP (“reporter” or “amplifier”) were injected into the SSp regions of C57BL/6 mice (Figure 4A,C). The gene discussed is RIC8B; the disease is infection.