In the present study, LC-PUFA administration restored the renal function tests, suppressed the oxidative stress, and attenuated the apoptotic process induced by MTX with a reduction in BAX expression and upregulation in the expression of BCL-2, consistent with the previous findings of Li et al. [26], who concluded that maresin-1, a derivative from docosahexaenoic acid, showed anti-inflammatory, antioxidant, and antiapoptotic effects against lipopolysaccharide-induced acute kidney injury by suppressing NF-kappaB. The gene discussed is BAX; the disease is acute kidney injury.