Interestingly, ENO1 expression was higher in patients with lymph node metastasis than in those without (p < 0.05, Figure 1E) and the expression level of ENO1 was higher in the primary lesions of OSCC patients with pathologically positive lymph node metastasis by IHC staining (Figure S1B). Here, ENO1 is linked to metastatic malignant neoplasm in the lymph nodes.