To determine whether the increased migration of the Neogenin receptor expressing aggressive melanoma cells C8161 and Sk-Mel28 was due to Netrin-1 and Neogenin receptor interaction, we conducted a migration assay using C8161 or Sk-Mel28 aggressive melanoma cells that were incubated in the presence of SH-SY5Y and treated with either 5 μg/mL or 25 μg/mL of anti-Neogenin blocking antibody. The gene discussed is NEO1; the disease is melanoma.