Since Neogenin expression was shown to be higher in aggressive compared to poorly aggressive melanoma cells and that the ratio of Neogenin to Netrin-1 expression is also much greater for the aggressive compared to the poorly aggressive melanoma cells, we sought to determine whether the excess expression of Neogenin would translate in greater responsiveness to Netrin-1 in the aggressive melanoma cells. The gene discussed is NEO1; the disease is melanoma.