Furthermore, we demonstrated a significant decrease in pERK1/2 levels in C8161 aggressive melanoma cells treated with 5 μg/mL of anti-Neogenin blocking antibody (0.43 +/− 0.01-fold of control) and in Sk-Mel28 aggressive melanoma cells treated with 25ug/mL of anti-Neogenin blocking antibody (0.65 +/− 0.11-fold of control) (Figure 4C,D, p < 0.05). Here, NEO1 is linked to melanoma.