MX1 and infection: Because we wished to evaluate the effect of disassembly and reassembly of GFP-MxA condensates lasting several minutes to a few hours on VSV replication soon after initiation of the infection, the approach used required a single-cycle replication assay under conditions in which >90% of cells in a culture were infected (moi >10 pfu/cell), and not a low-multiplicity multiple-cycle virus assay.