These AlphaScreen results were then confirmed by transactivation assay performed in HepG2 cells, a liver cancer cell line, transiently co-transfected with the following vectors: hLIFR, hgp13,0 and pGL4.47[luc2P/SIE/Hygro] containing the gene for Luciferase under the control of the STAT3 inducible elements (SIE), and finally a vector for human Renilla gene as a control for transfection efficiency and cell viability. The gene discussed is STAT3; the disease is liver cancer.