In order to investigate whether the PI3K/Akt pathway is regulated in visfatin-induced promotion of PDGF-C expression, we treated chondrosarcoma cells with Ly294002 or wortmannin (PI3K inhibitors), an Akt1/2 inhibitor, or transfected the cells with the respective siRNAs. The gene discussed is PDGFC; the disease is chondrosarcoma.