ATG5 and hydrops fetalis: The mRNA and protein expression (Figure 8A–L) of the key autophagy regulating genes Beclin1, Atg5, Lc3-II, and Lamp-1 significantly decreased (p ≤ 0.05), whereas Sqstm1/p62 expression elevated in the ovary of the HFD-HF diet-fed rats, compared to control.