In the current study, the decreased expression of ATG5 and Beclin-1, as well as FSHR and aromatase; steroidogenic proteins StAR and 3βHSD; and E2 and P4 levels occurs, indicating that autophagy inhibition may have been involved in the defective differentiation of the ovarian follicles of adult rats fed with HFD-HF diet. This evidence concerns the gene ATG5 and hydrops fetalis.