Thus, we hypothesized that “pre-pubertal exposure to HFD-HF diet may interfere with the specific proteins that regulate pituitary gonadotropins and key autophagy biomarkers leading to defective ovarian follicular growth and differentiation in adult rats.” In this investigation, we show that HFD-HF diet reduced GnRHR on pituitary gonadotropes, which resulted in decreased gonadotropins level as well as a disruption of a hormonal interplay of gonadotropins regulation. Here, GNRHR is linked to hydrops fetalis.