In a comparative study, to understand the chemotoxic potential of Berberine on lung cancer cells with different p53 statuses, Berberine treatment was tested against two different human NSCLC cancer cell lines, A549 with wild-type p53 (p53+/+) and p53-deficient H1299 cells (p53−/−). The gene discussed is TP53; the disease is lung carcinoma.