Indeed, when the levels of Tregs were determined in patients, higher percentages of Foxp3 populations could be seen in the PBMCs, and when naïve CD4+ T cells were differentiated to Tregs even though decreased numbers of expanded Tregs could be seen in ALS patients, they secreted higher levels of IL-10 when compared to the healthy controls. This evidence concerns the gene CD4 and amyotrophic lateral sclerosis.