These cells were able to express known mesoderm markers (BRACH, MIXL1 and MSGN), and with the replacement of the second myogenic differentiation medium (M2) during 6 to 8 days, the cells acquired the typical phenotype of myoblasts, by showing expression of PAX3, PAX7 and MYOD transcription genes for muscle lineage commitment, with no significant differences between the two HSP genotypes and the CTRL ones. The gene discussed is MIXL1; the disease is hereditary spastic paraplegia.