Ginesta et al. [36] assessed the methylation status of EN-1, HRH2, SPARC, APC and CDH13 gene promoters with pancreatic juice cfDNA and indicated that APC methylation was detected in ampullary carcinoma (76%) and IPMN (80%) but was occasionally observed in CP (7%). Here, HRH2 is linked to carcinoma of the ampulla of vater.