To characterize effects of PHF14 depletion on tumor growth in vivo, we inoculated PHF14 knockout (sgRNA-PHF14) SHSY-5Y cells and SHSY-5Y control cells subcutaneously into five-week-old male athymic nude mice (Nu/J, n = 10) at the density of 2 × 106 cells/animal. Here, PHF14 is linked to neoplasm.