In the models where the IHC markers were evaluated without tissue segmentation but HPV status was included, HPV RNA positivity, higher number of all PD-1+CD8+ T cells, and lower tumor stage or nodal status were connected with better OS and DSS, and low number of all CTLA4+CD8+ T cells was linked to better OS (Table 4 and Supplement Table S3). This evidence concerns the gene CTLA4 and neoplasm.