In addition, bioinformatics analysis suggested that nine DEGs (including SLC18A2, SNAP25, CACNA1B, GABRG2, DRD3, CAMK2A, NR5A1, and STAR) identified from neuroactive ligand-receptor interaction, dopaminergic synapse, synaptic vesicle cycle, cholinergic synapse, and Cushing syndrome pathways were crucial for the semen volume of drakes. This evidence concerns the gene NR5A1 and Cushing syndrome due to macronodular adrenal hyperplasia.