In addition, bioinformatics analysis suggested that nine DEGs (including SLC18A2, SNAP25, CACNA1B, GABRG2, DRD3, CAMK2A, NR5A1, and STAR) identified from neuroactive ligand-receptor interaction, dopaminergic synapse, synaptic vesicle cycle, cholinergic synapse, and Cushing syndrome pathways were crucial for the semen volume of drakes. Here, CACNA1B is linked to Cushing syndrome.