By performing Cut&Tag-qPCR assay with 3 different pairs of primers of Mdm2 promoter, we found that inhibition of Wdr5 decreased the enrichment of H3K4 trimethylation at the promoter of Mdm2 compared to the control groups, which is previously indicated in a study of neuroblastoma [33] Therefore, upregulated Mdm2 resulted in increased p53 and p21 expression, and finally led to cell senescence. Here, MDM2 is linked to neuroblastoma.