Compared to the commercial media, breast cancer cell line 231BR cultivated in Plasmax exhibited the inhibition of integrin signaling, and PAK signaling pathways, leading to the decrease in cell invasion and cell death, while the activation of unfolded protein response signaling and 14-3-3 mediated signaling pathways in brain cancer cell line CRL are associated with enhanced protein synthesis, cellular migration and invasion. Here, YWHAQ is linked to brain cancer.