In order to assess any differences in Ras/RAF/MEK/ERK signaling between A375 melanoma models in 2D versus 3D tissue culture, WT, NRAS Q61K, KRAS G13D, and MEK1 Q56P A375 cells were grown in either 2D or 3D tissue culture then total cellular protein was harvested for immunoblotting. Here, KRAS is linked to melanoma.