Tumor mutational burden (TMB) was correlated with response to immune checkpoint inhibitors (ICIs) in a retrospective cohort, including 1661 patients treated with ICIs, that could be either monoclonal antibody directed against programmed cell death protein-1 or monoclonal antibody directed against programmed cell death ligand 1 (anti-PD-1/anti-PD-L1), monoclonal antibody directed against cytotoxic T lymphocyte-associated antigen (anti-CTLA-4) or a combined treatment regimen including one anti-PD-1/anti-PD-L1 and one anti-CTLA-4 (ICIs combination). The gene discussed is CD274; the disease is neoplasm.