In this study, we detected the expression of pERK1/2 in the cytoplasm and nucleus of CRC cells and observed that overexpression of SUN5 increased the nuclear accumulation of phosphorylated ERK1/2, with Lamin B1 as the internal reference in the nucleus and GAPDH as the internal reference in the cytoplasm (p < 0.05, Figure 8A). The gene discussed is GAPDH; the disease is colorectal carcinoma.