BCL2 and breast cancer: Further, we analyzed protein and mRNA expressions of BCL-2 by western blotting and qPCR for nine cancer cell lines belonging to lung (A549, NCI-H460, and NCI-H1299), cervical (HeLa), colon (HCT-116), and breast cancers (BT474, ZR-75, MCF7, and MDA-MB-231).