Based on the role of PGK1 in remolding the immune microenvironment, as indicated above, we further used the Peking University database (http://lung.cancer-pku.cn/index.php, accessed on 18 May 2022)—which includes deep single cell transcriptome data as well as complete T cell receptor relevant data, portraying the multi-dimensional characteristics of NSCLC-infiltrating lymphocytes—in order to evaluate PGK1 expression in global T cells of non-small cell lung cancer at the single-cell level. This evidence concerns the gene PGK1 and non-small cell lung carcinoma.