Considering the effect on TME, in murine models, the comparison of CFRT with HFRT revealed that the latter fractionation schedule may inhibit hypoxia and reduce the recruitment of immunosuppressive cells into primary tumors, generating a microenvironment with lower PD-L1 levels and boosting not only local, but also systemic CD8- mediated anticancer immunity [30]; although, a large number of studies showed that high-dose/fraction irradiation may alter tumor blood vessels, increasing tumor hypoxia with the recruitment of immunosuppressive cells into tumors and a PDL-1 increase [31,32]. The gene discussed is CD8A; the disease is neoplasm.