In addition, Western blot analysis showed that the upregulated phosphorylation of TBK1 and NF-κB was almost fully reversed by C-176 (Figure 3C,D), indicating that antagonist C-176 treatment can inhibit the STING signaling pathway in the mPFC of CIBP rats, which may be the reason for the pain and anxiety-like behavior relief, further confirming STING may exert a pain-promoting effect in the mPFC of CIBP rats. This evidence concerns the gene NFKB1 and Anxiety.