Puerarin can not only regulate serum enzymes, reduce TGF-β1 production, and inhibit excessive collagen deposition by inhibiting TNF-α/NF-κB (24), PI3K/Akt (25), and TGFβ1/Smad (26) signaling pathways, but can also reduce liver fibrosis by inhibiting excessive collagen deposition. This evidence concerns the gene TGFB1 and Hepatic fibrosis.